Terbium(III) Luminescence-Based Assay for Esterase Activity

Luminescence-based Assay Methods in Drug Discovery

4 Luminescence-based assay methods in drug discovery

Optimization of a Microplate-Based Assay to Assess Esterase Activity in the Alga Pseudokirchneriella subcapitata

The present work describes the optimization of a short-term assay, based on the inhibition of the esterase activity of the alga Pseudokirchneriella subcapitata, in a microplate format. The optimization of the staining procedure showed that the incubation of the algal cells with 20 μmolL fluorescein diacetate (FDA) for 40 min allowed discrimination between metabolic active and inactive cells. Th...

A Sensitive Neutralization Assay for Influenza C Viruses Based on the Acetylesterase Activity HEF Glycoprotein

Influenza C virus possesses specific neuraminate-O-acetylesterase as a receptor-destroying function. This enzymatic activity of the viral glycoprotein HEF (Hemagglutinin, esterase activity and fusion factor) can be visualized in situ by the use of distinct color substrates. Hereby the localization, as well as the quantity of synthesized HEF protein is detectable. We further developed the estera...

A luminescence-based assay of UDP-sugar producing pyrophosphorylases†

A continuous spectrophotometric assay for the determination of diamondback moth esterase activity.

Conventional methods to determine esterase activity from insects are composed of a three-step process where the enzyme is allowed to hydrolyze a 1-naphthyl acetate substrate, that reaction is quenched by a SDS detergent, and then a Fast Blue B dye complex is formed with 1-naphthol, the product of 1-naphthyl acetate hydrolysis. These methods measure dye-product complex rather than the product, 1...